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Board Certified Physician-Prescribed Peptides · Compounded by pharmacies in USA

The smarter way to celebrate.

Your body deserves
more than good
intentions.

One protocol designed to carry you through the weekend — and bring you back whole.

Protect your body and brain, and feel like it's your first time.

What a protocol can do is defend what recovery depends on — sleep, circadian signaling, antioxidant reserve — before the deficit compounds across seasons and your pathways weaken.

Semax for neurotrophic repair. Epitalon for circadian rhythm. BPC-157 and KPV for gut and tissue. GHK-Cu for cellular remodelling. Glutathione for repletion of antioxidants.

Protect. Enjoy. Restore. Celebrate well.

What Sequence is

Not a supplement stack.
A clinical peptide protocol.

Festival culture has changed. The people showing up today are professionals, athletes, founders — people who take their recovery as seriously as their performance. We built Sequence for them.

Every compound in the three-phase protocol is physician-prescribed, pharmacy-compounded, and evidence-informed. The Pre stack primes you. The During stack sustains you. The Post stack restores you. Nothing more, nothing less.

The Process

Three steps to
the full protocol.

01
Health intake

A thorough questionnaire — medications, history, goals. A clinician reviews everything before a single compound is considered.

02
Physician prescription

A licensed physician writes the prescription. Everything is compounded to order from a US facility.

03
Delivered, labelled, ready

Your kit arrives with dosing schedules, protocols, syringes, wipes and direct access to your care team.

Phase 01 — Pre-Festival

Arrive already ahead.

We have paired several medications together to minimize injections.

(Maximum of 2 injections per stage with one nasal delivery)

Start 7–14 days before the event. Build your baseline before the weekend demands one.

Peptides — Physician-prescribed

BPC-157
250–500 mcg · Subcutaneous · Daily

Body Protection Compound. Supports gut lining integrity and systemic resilience ahead of sustained load. Primes connective tissue and modulates neurotransmitter pathways before the weekend begins.

GutRecoveryResilience
Epitalon
5–10 mg · Subcutaneous · Daily

A tetrapeptide that acts on the pineal gland to regulate melatonin and stabilise circadian rhythm. Sleep quality in the lead-up is everything — Epitalon sets the foundation before the disruption starts.

SleepCircadianLongevity
Semax
500–1000 mcg · Intranasal · Daily

ACTH-derived nootropic. Elevates BDNF, sharpens focus, builds stress resilience without stimulant load. Cognitive priming for environments that demand full presence from the first moment you arrive.

CognitionBDNFFocus
GHK-Cu
1–2 mg · Subcutaneous · Daily

Copper-binding tripeptide found naturally in plasma. Activates over 4,000 genes linked to repair, antioxidant defence, and anti-inflammatory signaling. Builds the baseline your body draws on all weekend.

AntioxidantRepairSkin
TB-500
2 mg · Subcutaneous · Weekly

Held at maintenance alongside BPC-157. Where BPC-157 works locally on the gut, TB-500 works systemically — sustaining circulation and limiting the inflammatory cascade across muscle and connective tissue under repeated days of physical load.

SystemicCirculationTissue
KPV
500 mcg · Subcutaneous · Daily

The C-terminal tripeptide of alpha-MSH. Carries the anti-inflammatory signaling of the parent hormone without its pigmentary effects. Started early to lower baseline inflammatory tone and reinforce gut barrier function alongside BPC-157.

Anti-InflammatoryGutImmune

Supplements — Daily, from 14 days prior

Magnesium Glycinate
400 mg · Before bed
Sleep quality, muscle relaxation, nervous system priming.
Omega-3 (EPA/DHA)
2–3 g · With food
Anti-inflammatory baseline and cell membrane integrity.
Vitamin D3 + K2
5,000 IU D3 / 100 mcg K2
Immune modulation, bone and cardiovascular support.
Phase 02 — During Festival

Stay sharp. Stay whole.

Active use throughout the event. Sustain the baseline, protect what matters, sleep whenever you can.

Peptides — Active protocol

Semax
300–600 mcg · Intranasal · Morning

Maintained through the event for sustained mental clarity and alertness. No stimulant mechanism, no adrenal cost. Just cleaner thinking when the environment is doing everything it can to cloud it.

ClarityAlertnessNeuroprotection
KPV
500 mcg · Subcutaneous · Daily

α-MSH-derived tripeptide with potent anti-inflammatory and gut-protective properties. Keeps the GI tract calm when eating is irregular and the body is under sustained environmental stress.

Anti-InflammatoryGutImmune
BPC-157
250 mcg · Subcutaneous · Maintenance

Held at maintenance dose to sustain gut integrity and neurotransmitter balance throughout. The quiet backbone of the During stack — working in the background so you don't have to think about it.

MaintenanceGutNeuro
TB-500
2 mg · Subcutaneous · Weekly

Held at maintenance alongside BPC-157. Where BPC-157 works locally on the gut, TB-500 works systemically — sustaining circulation and limiting the inflammatory cascade across muscle and connective tissue under repeated days of physical load.

MaintenanceCirculationAnti-Inflammatory

Supplements — Throughout the event

Electrolyte Complex
Per label · 2–3× daily
Sodium, potassium, magnesium. Non-negotiable.
Activated Charcoal
1–2 g · As needed
GI binder. Take well away from all other compounds.
CoQ10 (Ubiquinol)
200 mg · Daily with fat
Mitochondrial energy. Antioxidant protection under load.
Phase 03 — Post-Festival

Come back all the way.

Begin immediately after the event. Continue 7–14 days. This is where most protocols stop short — Sequence doesn't.

Peptides — Recovery protocol

BPC-157
500 mcg · Subcutaneous · Reload

Back to full dose. Accelerates systemic recovery — gut mucosal healing, neurotransmitter rebalancing, tissue repair from the weekend's accumulated load. The hardest-working compound in the Post stack.

ReloadGut RepairCNS
Epitalon
5 mg · Subcutaneous · 5-day burst

A short burst post-event to restore melatonin regulation and repair sleep architecture. Counters the oxidative acceleration from extended environmental exposure. Resets the clock — properly.

Sleep ResetAntioxidantCircadian
GHK-Cu
2 mg · Subcutaneous · 10 days

Elevated post-event to maximise skin regeneration and antioxidant gene expression. The damage from UV, dehydration, and oxidative stress doesn't announce itself — GHK-Cu addresses it whether you feel it or not.

RegenerationCollagenAntioxidant
TB-500
2.5 mg · Subcutaneous · Twice weekly · 2 weeks

Paired with BPC-157 through the recovery window. Studied for accelerated wound healing and reduced fibrotic scarring — the difference between tissue that repairs and tissue that repairs badly. Angiogenesis restores perfusion to what the weekend depleted.

RepairAnti-FibroticPerfusion
KPV
500 mcg · Subcutaneous · Daily · 2 weeks

Held through the recovery window. Acts on NF-κB signaling to damp the inflammatory cascade, with particular activity across the gastrointestinal tract — the tissue that took the most sustained load.

Anti-InflammatoryGut RepairImmune

Supplements — Recovery phase

Milk Thistle (Silymarin)
500 mg · Twice daily · 2 weeks
Hepatoprotection and liver enzyme normalisation.
Magnesium Glycinate
400–600 mg · Before bed
Nervous system recovery and deep sleep facilitation.
Glutathione (Injectable)
500 mg · Daily · 7 days
Direct repletion of the master antioxidant depleted by alcohol, stimulants, and sleep loss.
Get the full Protocol

Full Protocol

Everything, mapped.

Compound Pre-Festival During Post-Festival Primary role
BPC-157LoadingMaintenanceReloadGut repair, recovery, neuromodulation
TB-5002 mg weeklyMaintenance2.5 mg · 2×/wkSystemic repair, circulation, anti-fibrotic
KPV500 mcg dailyYesDaily · 2 weeksAnti-inflammatory, gut, immune
EpitalonYes5-day burstSleep, circadian rhythm, antioxidant
SemaxFull doseReducedCognition, BDNF, neuroprotection
GHK-CuYesElevated · 10dAntioxidant genes, regeneration, skin
Glutathione (Injectable)500 mg · 7 daysMaster antioxidant repletion, detox
Magnesium Glycinate400 mg400–600 mgSleep, muscle, nervous system
Omega-3 (EPA/DHA)2–3 gAnti-inflammatory baseline, membranes
Vitamin D3 + K25,000 IU / 100 mcgImmune modulation, bone, cardiovascular
Electrolyte Complex2–3× dailyHydration, sodium/potassium/magnesium
Activated CharcoalAs neededGI binder, taken away from other compounds
CoQ10 (Ubiquinol)200 mgMitochondrial energy, antioxidant
Milk Thistle (Silymarin)500 mg · 2×/dayHepatoprotection, liver enzyme support
Pre-FestivalLoading dose
DuringMaintenance
Post-FestivalReload
Gut repair, recovery, neuromodulation
Pre-Festival2 mg weekly
DuringMaintenance
Post-Festival2.5 mg · twice weekly
Systemic repair, circulation, anti-fibrotic
Pre-Festival500 mcg daily
DuringYes
Post-FestivalDaily · 2 weeks
Anti-inflammatory, gut, immune
Pre-FestivalYes
During
Post-Festival5-day burst
Sleep, circadian rhythm, antioxidant
Pre-FestivalFull dose
DuringReduced
Post-Festival
Cognition, BDNF, neuroprotection
Pre-FestivalYes
During
Post-FestivalElevated · 10 days
Antioxidant genes, regeneration, skin
Pre-Festival
During
Post-Festival500 mg daily · 7 days
Master antioxidant repletion, detox
Pre-Festival400 mg
During
Post-Festival400–600 mg
Sleep, muscle recovery, nervous system
Pre-Festival2–3 g daily
During
Post-Festival
Anti-inflammatory baseline, membranes
Pre-Festival5,000 IU D3 / 100 mcg K2
During
Post-Festival
Immune modulation, bone, cardiovascular
Pre-Festival
During2–3× daily
Post-Festival
Hydration, sodium/potassium/magnesium
Pre-Festival
During1–2 g as needed
Post-Festival
GI binder, away from other compounds
Pre-Festival
During200 mg daily
Post-Festival
Mitochondrial energy, antioxidant
Pre-Festival
During
Post-Festival500 mg twice daily · 2 weeks
Hepatoprotection, liver enzyme support

Physician-prescribed. Pharmacy-compounded. Delivered before the weekend starts.

Start Your Protocol
/ Sequence RX Education · Body Systems Reference

The body under
pressure.

A biochemical breakdown of what happens inside the body after a night involving these substances — from neurotransmitter depletion to gut barrier compromise and cell repair shutdown.

Serotonergic Dissociative Stimulant Depressant Sleep Deprivation Dehydration
Serotonergic
/ Psychedelic
Dissociative
Stimulant
Depressant
Lifestyle
Stressor
01 — 08
01 / Entactogen · Stimulant
MDMA (Ecstasy / Molly)
Serotonergic
Neurotransmitters
Serotonin is catastrophically depleted. MDMA forces neurons to dump their entire serotonin supply at once. The day after, reserves are nearly empty — producing flat mood, emotional blunting, depression, and anxiety lasting 3–7 days (the "comedown").
Cell Health
Free radical cascade damages cells across organs. MDMA metabolism produces reactive oxygen and nitrogen species that damage mitochondrial proteins, lipids, and DNA in the brain, liver, and heart. Even a single dose causes measurable DNA strand breaks.
Cell Regeneration
Glutathione — the body's master antioxidant — is significantly depleted. This strips the cell's main defence against oxidative damage and halts tissue repair across the body.
Gut Health
Gut-brain serotonin axis disrupted. ~90% of the body's serotonin is produced in the gut. Depletion impairs motility, digestion, and gut-brain signaling — causing nausea, cramping, and appetite loss for days after use.
Immune System
Immune suppression via hyperthermia and oxidative stress. Body resources are redirected to damage control, leaving you more vulnerable to illness in the 24–48 hours post-use.
Hormones
Cortisol spikes, testosterone drops. The HPA stress axis activates acutely. Anabolic hormones dip — impairing recovery, mood stability, and muscle repair.
02 / Classic Psychedelic
Psilocybin (Magic Mushrooms)
Serotonergic
Neurotransmitters
5-HT2A receptors saturated then silenced. Psilocin floods serotonin receptors in the prefrontal cortex. The brain downregulates receptor sensitivity as defence — leaving mild serotonin signal blunting, fatigue, or emotional flatness the day after.
Cell Regeneration
Neuroplasticity is promoted, not suppressed. Psilocybin stimulates BDNF (brain-derived neurotrophic factor), encouraging new neural connections. It does not significantly suppress cellular repair — a key distinction from stimulants.
Gut Health
Minimal gut disruption. Serotonin receptor activation in the gut can cause nausea during the experience, but psilocybin doesn't cause the sustained gut damage seen with alcohol or stimulants. Microbiome largely intact next day.
Immune System
Anti-inflammatory signal noted in research. Studies show inflammatory markers like CRP may drop 48 hours after use. The immune system is not significantly suppressed after a single experience.
Hormones
Cortisol spikes acutely — especially in difficult trips — then returns to baseline within hours. Growth hormone and melatonin are less disrupted than with stimulants, so sleep quality the following night recovers relatively quickly.
03 / Dissociative Anaesthetic
Ketamine
Dissociative
Neurotransmitters
Glutamate system destabilised — then rebounds hard. Blocking NMDA receptors triggers a compensatory glutamate surge after the drug clears. The prefrontal cortex and hippocampus are flooded with excitatory signals, causing brain fog, memory impairment, and difficulty concentrating the next day.
Cell Regeneration
Mixed picture. Ketamine disrupts hippocampal synaptic signaling, impairing memory consolidation — a cell-level process. It also upregulates BDNF, which may partially offset this. Repeated use accelerates neurotoxicity significantly.
Gut / Bladder
Urinary tract stressed from the first use. Ketamine metabolites irritate the epithelial lining of the bladder and urinary tract. Repeated use causes progressive irreversible bladder damage — ketamine cystitis — a defining long-term risk.
Immune System
Neuroinflammation risk from glutamate rebound. The post-drug glutamate surge can trigger inflammatory signaling in the brain. Without clinical monitoring, this neuroinflammation may persist well into the next day.
Hormones
Norepinephrine spikes during use, then crashes. The cardiovascular stimulation from norepinephrine release is followed by a relative deficit — fatigue and low blood pressure can persist into the following day. Cortisol remains elevated for several hours post-use.
04 / Stimulant
Cocaine
Stimulant
Neurotransmitters
Dopamine floods synapses, then craters below baseline. Cocaine blocks dopamine reuptake creating intense euphoria — then the cleanup leaves dopamine well below normal. The day after brings anhedonia (inability to feel pleasure), depression, fatigue, and intense cravings.
Cell Health & DNA
Significant DNA and mitochondrial damage from a single dose. Cocaine causes DNA strand breaks in brain, liver, and blood cells. Free radicals damage mitochondrial proteins, impairing the cell's ATP (energy) production capacity at the most fundamental level.
Cell Regeneration
Vasoconstriction starves every tissue of oxygen. Cocaine constricts blood vessels systemically, reducing oxygen delivery to all organs — the exact opposite of what cells need to repair. Tissue regeneration is impaired across every organ system.
Gut Health
Blood flow to intestines sharply reduced. Vasoconstriction limits gut blood supply, impairing digestion and nutrient absorption. In heavy use, this can cause ischaemic bowel damage. The systemic stress response also disrupts the microbiome.
Immune System
Neutrophil and macrophage function impaired. The body's first-line defenders are compromised. Glutathione depletion strips antioxidant defences from cells, leaving the body vulnerable to infection and oxidative damage for 24–48 hours post-use.
Hormones
HPA axis dysregulation — cortisol persists, testosterone and HGH drop. This catabolic state is the opposite of recovery. All the signals needed for muscle repair, mood regulation, and cellular restoration are suppressed simultaneously.
05 / CNS Depressant
Alcohol
Depressant
Neurotransmitters
GABA amplified, glutamate blocked — then the rebound. Alcohol boosts inhibitory GABA and suppresses excitatory glutamate. The hangover is partly the system snapping back: glutamate overactivates, causing anxiety, light/sound sensitivity, and restlessness.
Cell Regeneration
HGH (Human Growth Hormone) release almost entirely blocked. HGH — the body's primary cell repair signal — is released almost exclusively during deep slow-wave sleep. Alcohol destroys deep sleep architecture, meaning HGH secretion is severely blunted. Cell and tissue repair across the entire body stalls for the night of drinking and the following night.
Gut Health
Leaky gut triggered within hours — even from one binge. Alcohol damages tight junctions in the intestinal wall, the molecular seals between gut cells. Bacterial toxins (endotoxins) pass from the gut into the bloodstream triggering systemic inflammation. Beneficial bacteria (Lactobacillus, Bifidobacterium) are killed off; harmful strains proliferate.
Liver / Acetaldehyde
Liver converts alcohol → acetaldehyde (highly toxic) → acetate. Acetaldehyde causes nausea, flushing, headache, and directly damages liver cells. Glutathione stores are depleted attempting to neutralise it, stripping cellular antioxidant defences.
Immune System
Neutrophil function impaired for up to 24 hours. Even a single binge disrupts white blood cells responsible for fighting bacterial infection. Endotoxins leaking from the gut simultaneously trigger a systemic inflammatory response — a double hit on immune function.
Hormones
Cortisol up. Testosterone, LH, and HGH down. This catabolic state persists for 12–24 hours post-drinking. Everything needed for rebuilding — muscle recovery, mood stability, cellular restoration — is simultaneously suppressed.
06 / Classic Psychedelic
LSD (Acid)
Serotonergic
Neurotransmitters
5-HT2A receptors oversaturated for 8–12 hours. LSD binds serotonin receptors far more tightly and for much longer than psilocybin. The brain compensates by downregulating receptor density — the day after, serotonin signaling is blunted, contributing to post-trip fatigue or emotional flatness ("afterglow").
Cell Regeneration
REM sleep entirely suppressed for the night of use. LSD eliminates REM sleep — the phase critical for memory consolidation, protein synthesis, and cellular repair. The body misses a complete repair cycle. This is one of the most significant physical costs of a full LSD experience.
Gut Health
Mild serotonergic disruption — appetite significantly suppressed. Gut serotonin receptor activation causes nausea during the experience. Appetite suppression can persist for 12–24 hours after, reducing nutrient intake during a period when the body most needs fuel for repair.
Immune System
Sustained norepinephrine-driven stress response. LSD triggers norepinephrine release, keeping the sympathetic nervous system activated for the duration of the trip. This prolonged stress state moderately taxes immune resources, though direct immune suppression is less severe than stimulants.
Hormones
Cortisol elevated throughout. Melatonin significantly suppressed. Melatonin — essential for sleep onset and acting as a powerful cellular antioxidant — is blocked during the experience. This compounds the cell repair deficit from missed sleep cycles.
07 / Lifestyle Stressor
Sleep Deprivation
Lifestyle
Neurotransmitters
Adenosine builds up — GABA falls. Adenosine (sleep pressure chemical) accumulates throughout the night and cannot clear without sleep — causing cognitive fog, slowed reaction times, and impaired working memory. GABA (the calming neurotransmitter) decreases, causing agitation and emotional dysregulation.
Cell Regeneration
HGH secretion stops. DNA damage increases by ~139%. Growth hormone is released almost exclusively during deep slow-wave sleep. One missed night means zero HGH pulse — tissue repair, muscle synthesis, and skin regeneration all stall. A single night of total sleep deprivation measurably depletes ATP (cellular energy) and glutathione (antioxidant defence).
Gut Health
Gut microbiome dysregulated within 24 hours. Sleep and the gut microbiome share a bidirectional biological clock. Even one poor night alters gut bacteria composition, reduces microbial diversity, and impairs motility — causing digestive discomfort and reduced nutrient absorption the next day.
Immune System
Pro-inflammatory cytokine cascade — IL-6, IL-1β, TNF-α all spike. The anti-inflammatory cytokine IL-10 simultaneously drops. The body enters a low-grade inflammatory state within hours of sleep loss. White blood cell composition is measurably altered by morning.
Hormones
Cortisol elevated all day. Ghrelin up. Leptin down. Insulin sensitivity drops. Hunger hormone surges while the satiety signal collapses — driving intense food cravings. Insulin sensitivity decreases, mimicking a pre-diabetic metabolic state. All from a single missed night.
08 / Lifestyle Stressor
Dehydration
Lifestyle
Neurotransmitters
Serotonin and dopamine production drops before physical symptoms appear. Both neurotransmitters require water for synthesis and synaptic transport. Mild dehydration reduces their production rate — causing irritability and low motivation that is routinely misattributed to mood rather than hydration status.
Cell Regeneration
Every biochemical repair process slows. Water is required for enzyme activity, nutrient transport, waste removal, and protein synthesis. Dehydration concentrates metabolic waste inside cells, creating a hostile environment for division and repair — the cellular equivalent of trying to clean a house with no running water.
Gut Health
Intestinal mucus layer thins rapidly. This mucus is the primary protection for the gut wall and the main food source for beneficial bacteria. When it thins, gut motility slows (contributing to constipation), microbial diversity decreases, and the barrier against harmful infiltration weakens.
Immune System
Lymphatic system impaired — immune cell trafficking slows. The lymphatic system transports white blood cells and clears cellular debris. It depends entirely on adequate fluid volume. Dehydration slows lymph flow, reducing both the speed and effectiveness of immune response throughout the body.
Hormones & Electrolytes
Cortisol spikes. ADH and aldosterone surge to retain fluid. Sodium, potassium, and magnesium shift out of balance, disrupting nerve signal transmission and muscle contraction at just 2% body water loss. Cerebral blood flow measurably drops — impairing executive function and decision-making.
3
The Glutathione Problem
MDMA, cocaine, and alcohol all independently deplete glutathione — the body's master antioxidant. Use all three in one night and antioxidant reserves can be nearly eliminated, leaving cells essentially unprotected during the critical repair window.
0
HGH Pulses After Alcohol + Sleep Loss
Human growth hormone — which drives all tissue repair and cell regeneration — is released almost entirely during deep sleep. Alcohol destroys deep sleep architecture. Miss sleep on top of that and the body receives zero repair signals for the entire night.
2%
Dehydration Threshold for Brain Impairment
Cerebral blood flow measurably drops and executive function begins to impair at just 2% body water loss — a level easily reached before thirst is even triggered. Serotonin and dopamine production decrease before any physical symptom is felt.

Celebrate well.

Start Your Protocol
Sequence RX

Physician-formulated peptide and supplement protocols for wellness. Medical-grade, prescription-only, compounded by pharmacies in the USA.

Sequence protocols are for informational purposes only and do not constitute medical advice. All peptide therapies require a valid physician prescription through Sequence RX.

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